Mouse Anti-Human IgM antibody (HRP)

• Catalog Number: orb44861
• Category: Antibodies
• Description: Mouse Monoclonal to Human IgM.
• Clonality: Monoclonal
• Clone Number: CH2
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Purified human IgM.
• Concentration: 1 mg/ml
• Dilution range: Western blotting: Recommended dilution: 1 μg/ml.Flow cytometry: Recommended dilution: 1-4 μg/ml, extracellular and intracellular staining.
• Purity: Purified antibody is conjugated with activated horseradish peroxidase (HRP) of high specific enzyme activity under optimum conditions and unconjugated antibody and free HRP are removed by size-exclusion chromatography.
• Conjugation: HRP
• Target: IgM
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 0.01% thimerosal
• Alternative names: Anti-IgM antibody
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 1 μg/ml. Flow cytometry: Recommended dilution: 1-4 μg/ml, extracellular and intracellular staining.
• Expiration Date: 12 months from date of receipt.

Western blotting detection (reducing conditions) of IgM in human plasma using anti-human IgM (CH2) peroxidase conjugate.

ELISA analysis of IgM in human serum using capture antibody MA2 and HRP-conjugated detection antibody CH2. Three different dilutions of human serum were used, and 10% BSA as negative control.