Mouse Anti-Human IgG (Fc) antibody

• Catalog Number: orb44867
• Category: Antibodies
• Description: Mouse Monoclonal to Human IgG (Fc).
• Clonality: Monoclonal
• Clone Number: EM-07
• Tested applications: ELISA, FC, ICC, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Fusion protein of human IgG Fc fragment.
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: IgG (Fc)
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-IgG (Fc) antibody
• Note: For research use only
• Application notes: Western blotting and ELISA: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments. The antibody EM-07 does not crossreact with IgM.
• Expiration Date: 12 months from date of receipt.

Western blotting detection of IgG κ light chain (1), IgG λ light chain (2) and IgG Fc fragment (3) purified from human serum, with MEM-09 antibody against IgG κ light chain (A) and EM-07 antibody against IgG Fc fragment (B).

Flow cytometry multicolor surface staining of human peripheral whole blood stained using anti-human IgG (Fc) (EM-07) purified antibody (concentration in sample 1 µg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 µl reagent / 100 µl of peripheral whole blood).

Separation of human monocytes (red-filled) from CD3 negative IgG (Fc) positive lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human IgG (Fc) (EM-07) purified antibody (concentration in sample 1 µg/ml, GAM APC). The pattern varies depending on the blood donor.

Flow cytometry surface staining pattern of human peripheral whole blood using anti-human IgG (Fc) (EM-07) purified antibody (concentration in sample 1 µg/ml, GAM APC). The pattern varies depending on the blood donor.

Flow cytometry surface staining pattern of human peripheral whole blood using anti-human IgG (Fc) (EM-07) purified antibody (concentration in sample 1 µg/ml, GAM APC). The pattern varies depending on the blood donor.