Mouse Anti-Human IgE antibody (FITC)

• Catalog Number: orb44856
• Category: Antibodies
• Description: Mouse Monoclonal to Human IgE.
• Clonality: Monoclonal
• Clone Number: BE5
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Purified human IgE.
• Concentration: 0.1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 5 μg/ml.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: IgE
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-IgE antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 5 μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis of basophil activation upon stimulation of normal (heparin-treated) whole blood with combination of IL-3 and Goat anti-IgE polyclonal antibody. Combination of anti-human IgE (BE5) FITC and anti-human CD63 (MEM-259) PE-Dyomics 647 was used (analysis in basophil window). A - staining of non-stimulated (control) sample; 1B - staining of IgE stimulated sample.