Mouse anti-CD68 Recombinant Monoclonal Antibody

• Catalog Number: orb1520128
• Category: Antibodies
• Description: CD68 Antibody
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: KP-1
• Tested applications: FC, IF, IHC
• Reactivity: Human
• Immunogen: Lysosomal fraction of human lung macrophages
• Antibody Type: Primary Antibody
• Concentration: 50 µg/ml
• Dilution range: Flow-Cyt Fixed in 4% formaldehyde and permeabilized with 50% methanol. 2 µl per 1 x 10^6 cells. Immunohistochemistry (IHC) 1:100 - 1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Multiplex Immunofluorescence (mIF) 1:250
• Form/Appearance: Whole IgG
• Purity: Recombinant antibody was purified from cell culture supernatant
• Conjugation: Unconjugated
• Target: CD68
• UniProt ID: P34810
• NCBI: NP_001242.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Phosphate Buffered Saline (PBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: LAMP4; scavenger receptor class D, member 1; SCARD
• Background: CD68 is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins.CD68 is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human CD68 in FFPE tonsil by IHC. Antibody: Mouse monoclonal anti-CD68. Secondary: HRP-conjugated goat anti-mouse IgG. Substrate: DAB

Detection of human CD68 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-CD68 antibody used at 1:100. Secondary: DyLight® 594-conjugated goat anti-mouse IgG

Detection of human CD68 in FFPE seminoma by IHC. Antibody: Mouse monoclonal anti-CD68. Secondary: HRP-conjugated goat anti-mouse IgG. Substrate: DAB. Counterstain:Hematoxylin (blue)

Detection of human CD68 (green) by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Mouse anti-CD68 monoclonal antibody used at 1:500. Secondary: HRP-conjugated goat anti-mouse IgG. Substrate: Opal™ . Counterstain: DAPI (blue).