Mouse Alpha-Melanocyte Stimulating Hormone (aMSH) ELISA Kit

SKU: orb779353

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Alpha-Melanocyte Stimulating Hormone(aMSH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-Melanocyte Stimulating Hormone(aMSH).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse aMSH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse aMSH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse aMSH in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 156.25-10000 pg/mL
• Sensitivity: 42 pg/mL
• Concentration: 10000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

α-MSH ELISA Kit, Intermedins ELISA Kit, Alpha-Melanotropin, Alpha-Melanocortin ELISA Kit, Alpha-Intermedin ELISA Kit