MonoMethyl-Histone H3 (Lys4) Antibody

• Catalog Number: orb1730700
• Category: Antibodies
• Description: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3
• Dilution range: IP: 1/20; WB: 1/500-1/1000; IHC: 1/50-1/100; ICC: 1/50-1/200
• Conjugation: Unconjugated
• MW: Calculated MW: 16 kDa; Observed MW: 16 kDa
• Entrez: 8290
• UniProt ID: Q16695
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Alternative names: H3K4me; H3 histone; HIST1H3A; Histone cluster 1; H
• Note: For research use only
• Expiration Date: 12 months from date of receipt.