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Catalog Number | orb386080 |
---|---|
Category | Antibodies |
Description | Moesin, a member of the talin-4.1 superfamily, is a linking protein of the sub-membranous actin cytoskeleton. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | MSN/491 |
Tested applications | FACS, IF, IHC-P, WB |
Reactivity | Human, Rat |
Isotype | Mouse IgG1, kappa |
Immunogen | Recombinant full-length human protein was used as the immunogen for the Moesin antibody. |
Antibody Type | Primary Antibody |
Dilution range | Flow cytometry: 1-2ug/million cells,Immunofluorescence: 2-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This Moesin antibody is available for research use only. |
UniProt ID | P26038 |
Storage | Store the Moesin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Titering of the Moesin antibody may be required for optimal performance. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human melanoma with Moesin antibody (clone MSN/491). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.
IHC testing of FFPE human placenta with Moesin antibody (clone MSN/491). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.
IHC testing of FFPE rat colon with Moesin antibody (clone MSN/491). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.
IHC testing of FFPE rat lung with Moesin antibody (clone MSN/491). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.
Western blot testing of human HT29 cell lysate with Moesin antibody (clone MSN/491). Predicted molecular weight ~68 kDa but routinely observed at 68-78 kDa.
SDS-PAGE Analysis of Purified, BSA-Free Moesin Antibody (clone MSN/491). Confirmation of Integrity and Purity of the Antibody.
Western blot testing of human PC3 cell lysate with Moesin antibody (clone MSN/491). Predicted molecular weight ~68 kDa but routinely observed at 68-78 kDa.
Flow cytometry testing of permeabilized human K562 cells with Moesin antibody (clone MSN/491); Red = isotype control, Blue = Moesin antibody.
Immunofluorescent staining of PFA-fixed human HeLa cells with Moesin antibody (clone MSN/491, green) and Reddot nuclear stain (red).