MOB1B Antibody

• Catalog Number: orb1263519
• Category: Antibodies
• Description: MOB1B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This MOB4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-206 amino acids from the C-terminal region of human MOB4A.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 25 kDa
• Target: MOB1B
• UniProt ID: Q7L9L4
• NCBI: Q7L9L4
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: MOB kinase activator 1B, Mob1 homolog 1A, Mob1A, M
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from K562, Daudi cell line (from left to right), using MOB4A Antibody at 1:1000 at each lane.

Western blot analysis in Daudi, K562 cell line lysates (35 ug/lane).

Fluorescent image of NIH/3T3 cell stained with MOB4A Antibody.NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MOB4A primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml).MOB4A immunoreactivity is localized to Cytoplasm significantly.

Formalin-fixed and paraffin-embedded human brain tissue reacted with MOB4A antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.