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Catalog Number | orb1473877 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to MMP14 |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | IF, WB |
Reactivity | Human |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MMP14. The exact sequence is proprietary. |
Dilution range | WB (1/500 - 1/1000) |
Form/Appearance | Mouse IgM. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | MMP14 |
Entrez | 4323 |
UniProt ID | P50281 |
Source | Mouse |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Mouse IgM. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | Matrix metalloproteinase-14; MMP-14; MMP-X1; Membr Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of MMP14 expression in A2058 (A), U251 MG (B), U87 MG (C) whole cell lysates. (Predicted band size: 65 kD; Observed band size: 65 kD)
Immunofluorescent analysis of MMP14 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
IF, IHC, WB | |
Human, Mouse, Rabbit | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
FC, IHC-P, WB | |
Bovine, Porcine, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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