MMP13 Antibody

• Catalog Number: orb1263949
• Category: Antibodies
• Description: MMP13 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This MMP13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 295-324 amino acids from the C-terminal region of human MMP13.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:500For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 54 kDa
• Target: MMP13
• UniProt ID: P45452
• NCBI: P45452
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Collagenase 3, 3424-, Matrix metalloproteinase-13,
• Note: For research use only
• Application notes: For WB starting dilution is: 1:500For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:500 dilution + MDA-MB-231 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of MMP13 using rabbit polyclonal MMP13 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MMP13 gene (Lane 2).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with MMP13 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.