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Catalog Number | orb1927719 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB24782 |
Tested applications | IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:500, WB: 1:1000, IHC-P: 1:50~100 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 46538 Da |
Target | This MMAA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-84 amino acids from the N-terminal region of human MMAA. |
UniProt ID | Q8IVH4 |
NCBI | NP_758454.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Methylmalonic aciduria type A protein, mitochondri Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of MMAA Antibody (N-term) in mouse liver tissue lysates (35 ug/lane). MMAA (arrow) was detected using the purified Pab.
Anti-MMAA Antibody (N-term) at 1:500 dilution + human liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
MMAA Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MMAA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.