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Catalog Number | orb866681 |
---|---|
Category | Antibodies |
Description | MLKL Rabbit Monoclonal Antibody |
Species/Host | Rabbit |
Clonality | Monoclonal |
Clone Number | 26M37 |
Tested applications | IP, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | A synthesized peptide derived from MLKL |
Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
Dilution range | WB 1:500-1:2000IP 1:50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 54 kDa |
UniProt ID | Q8NB16 |
Storage | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of MLKL using anti-MLKL antibody (orb866681). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HeLa whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human U87 whole cell lysates, Lane 7: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-MLKL antigen affinity purified monoclonal antibody (Catalog # orb866681) at 1: 1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90502) with Tanon 5200 system. A specific band was detected for MLKL at approximately 54 kDa. The expected band size for MLKL is at 54 kDa.
IP, WB | |
Mouse | |
Rabbit | |
Monoclonal | |
Unconjugated |
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