MLF1 Antibody (C-term)

• Catalog Number: orb1929857
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB18643
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 30627 Da
• Target: This MLF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 215-244 amino acids from the C-terminal region of human MLF1.
• UniProt ID: P58340
• NCBI: NP_001182363.1, NP_001123629.1, NP_001182362.1, NP_071888.1, NP_001123628.1, NP_001182361.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Myeloid leukemia factor 1, Myelodysplasia-myeloid
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

MLF1 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

MLF1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MLF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of MLF1 Antibody (C-term) in mouse heart tissue lysates (35 ug/lane). MLF1 (arrow) was detected using the purified Pab.

MLF1 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35 ug/lane). This demonstrates the MLF1 antibody detected the MLF1 protein (arrow).

Western blot analysis of MLF1 (arrow) using rabbit polyclonal MLF1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MLF1 gene.