MLANA Antibody

• Catalog Number: orb1274227
• Category: Antibodies
• Description: MLANA Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: MLANA/1761R
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG, k
• Immunogen: Human protein was used as the immunogen for this recombinant Melan-A antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1ug/10^6 cellsImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RTPrediluted IHC only format: incubate for 30 min at RT (1)The optimal dilution of the recombinant Melan-A antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: MLANA
• UniProt ID: Q16655
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A,
• Note: For research use only
• Application notes: Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1ug/10^6 cellsImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RTPrediluted IHC only format: incubate for 30 min at RT (1)The optimal dilution of the recombinant Melan-A antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing FFPE human skin with recombinant Melan-A antibody (clone MLANA/1761R). HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

IHC testing of FFPE human melanoma with recombinant Melan-A antibody (clone MLANA/1761R). HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.