MLANA Antibody

• Catalog Number: orb1252767
• Category: Antibodies
• Description: MLANA Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: M2-9E3
• Tested applications: ELISA, FC, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG2b, kappa
• Immunogen: Recombinant human Melan-A protein was used as the immunogen for this antibody.
• Concentration: 0.2 mg/mL
• Dilution range: ELISA: order BSA-free format for coatingFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIP: 0.5-1ug/500ug protein lysateIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Melan-A antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: MLANA
• UniProt ID: Q16655
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A,
• Note: For research use only
• Application notes: ELISA: order BSA-free format for coatingFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIP: 0.5-1ug/500ug protein lysateIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Melan-A antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin/paraffin human melanoma stained with MART-1 / Melan-A antibody (M2-9E3). Note cytoplasmic staining of cells.

Formalin/paraffin human melanoma stained with MART-1 / Melan-A antibody (M2-9E3). Note cytoplasmic staining of cells.

SDS-PAGE Analysis of Purified, BSA-Free Melan-A Antibody (clone M2-9E3). Confirmation of Integrity and Purity of the Antibody.