MLANA Antibody

• Catalog Number: orb1252556
• Category: Antibodies
• Description: MLANA Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MLANA/788
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Canine, Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: Recombinant human MLANA protein was used as the immunogen for the Melan-A antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Western blot: 0.5-1 ug/mlPrediluted format : incubate for 30 min at RT (2)Optimal dilution of the Melan-A antibody should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: MLANA
• UniProt ID: Q16655
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A,
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Western blot: 0.5-1 ug/mlPrediluted format : incubate for 30 min at RT (2)Optimal dilution of the Melan-A antibody should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human testis stained with Melan-A antibody (MLANA/788).

Formalin-fixed, paraffin-embedded human melanoma stained with Melan-A antibody (MLANA/788).