MKK1/2 (phospho-S218/222) antibody

• Catalog Number: orb224176
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MKK1/2 (Phospho-S218/222)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rabbit, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S218/222 of human MKK1/2 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MAP2K1; MAP2K2
• Entrez: 26395, 5604, 170851, 26396, 5605, 58960
• UniProt ID: P31938, P36507, Q63932, P36506, Q02750, Q01986
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti MAP2K1 antibody, anti MEK1 antibody, anti PRK
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MKK1/2 (Phospho-S218/222) expression in HeLa EGF-treated (A), A431 EGF-treated (B) whole cell lysates. (Predicted band size: 43; 44 kD; Observed band size: 45 kD)

Immunohistochemical analysis of MKK1/2 (Phospho-S218/222) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.