MIP-3 alpha Antibody

SKU: orb345242

Description

MIP-3 alpha antibody

Images & Validation

• Tested Applications: WB
• Dilution range: WB: 0.5µg/mL
• Reactivity: Mouse
• Application Notes:
  MIP-3α antibody has been tested for use in western blot and suitable for use in ELISA, IP, and Neutralization. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 4N5F7
• Immunogen: Anti-MIP-3α (RAT) Monoclonal Antibody was produced in rat by repeated immunizations with mature full length recombinant mouse MIP-3α produced in E.coli followed by hybridoma development.
• Purity: This product was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse MIP-3α protein. A BLAST analysis was used to suggest cross-reactivity with MIP-3α from mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with MIP-3α from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rat anti-MIP-3α antibody, CCL20, C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1

Western Blot of Mouse MIP 3α (RAT) antibody. Lane 1: mouse recombinant MIP-3a. Lane 2: human recombinant MIP-3a. Primary antibody: MIP 3α antibody at 0.1 ug/ml for overnight at 4°C. Secondary antibody: IRDye800™ goat anti-rat at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.

Quick Database Links

UniProt

O89093

RefSeq (Protein):NP_001153210.1