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MIP-3 alpha Antibody

SKU: orb345108

Description

MIP-3 alpha antibody

Images & Validation

Tested ApplicationsWB
Dilution rangeWB: 0.5µg/mL
ReactivityHuman
Application Notes
Anti-Human MIP-3α antibody has been tested by western blot and is suitable for use in ELISA, IP, and Neutralization. Specific conditions for reactivity should be optimized by the end user.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeIgG1
Clone No.3N2D9
ImmunogenAnti-MIP-3α (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human MIP-3α produced in E.coli followed by hybridoma development.
PurityAnti-MIP-3α was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human MIP-3α protein. A BLAST analysis was used to suggest cross-reactivity with MIP-3α from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with MIP-3α from other sources has not been determined.
ConjugationUnconjugated

Storage & Handling

StorageStore vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLiquid (sterile filtered)
Buffer/Preservatives0.01% (w/v) Sodium Azide
Concentration1.0 mg/mL
Dry Ice ShippingPlease note: This product requires shipment on dry ice. A dry ice surcharge will apply.
DisclaimerFor research use only

Alternative Names

mouse anti-MIP-3 alpha Antibody, mouse anti-MIP-3a Antibody, MIP-3α , CCL20, C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1

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MIP-3 alpha Antibody

Western Blot of Human MIP 3α (Mouse) Antibody. Lane 1: human recombinant MIP-3a. Lane 2: mouse recombinant MIP-3a. Primary antibody: Human MIP 3α antibody at 0.5 ug/ml for overnight at 4°C. Secondary antibody: IRDye800™ goat anti-mouse at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeq4759076

Documents Download

Datasheet
Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol

MIP-3 alpha Antibody (orb345108)

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