MGLL Antibody

• Catalog Number: orb1247258
• Category: Antibodies
• Description: MGLL Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-QDLPHLVNADGQY
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 35kDa band observed in Human Brain (Frontal Cortex) and Mouse Adipose lysates (calculated MW of 33.3kDa according to NP_001003794.1). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat for this particular batch.Immunohistochemistry:In paraffin embedded Human Liver, expression of MGLL is detected in hepatocytes. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: MGLL
• UniProt ID: Q99685
• NCBI: NP_001003794.1, NP_009214.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: monoglyceride lipase, HU-K5, MGLL, MGL, lysophosph
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 35kDa band observed in Human Brain (Frontal Cortex) and Mouse Adipose lysates (calculated MW of 33.3kDa according to NP_001003794.1). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat for this particular batch.Immunohistochemistry:In paraffin embedded Human Liver, expression of MGLL is detected in hepatocytes. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247258 (0.5 ug/ml) staining of Human Frontal Cortex (A) and Mouse Adipose (B) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247258 (5 ug/ml) staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

orb1247258 Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.