MGEA5 Antibody

• Catalog Number: orb1523170
• Category: Antibodies
• Description: MGEA5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 500 and 550
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: MGEA5
• UniProt ID: O60502
• NCBI: NP_036347.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: hyaluronidase in meningioma; MGEA5; OGA; nuclear c
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human MGEA5 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MGEA5 antibody orb1523170 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human MGEA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MGEA5 antibody orb1523170 used for IP at 6 µg per reaction.

Detection of human MGEA5 by immunohistochemistry.Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-MGEA5 (Cat. No. orb1523170) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).