METTL3 antibody

• Catalog Number: orb374082
• Category: Antibodies
• Description: Rabbit polyclonal antibody to METTL3
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein of human METTL3(NP_062826.2).
• Dilution range: WB: 1:500-2000, IHC-P: 1:100-200
• Conjugation: Unconjugated
• MW: 70KDa
• UniProt ID: Q86U44
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Alternative names: IME4, M6A, MT-A70, Spo8, hMETTL3, METTL3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Xiaodong Yu, Yaxun Li, Fanshuang Kong, Qun Xu METTL3 regulates FAM83D m6A modification to accelerate tumorigenesis of triple-negative breast cancer via the Wnt/β-catenin pathway Toxicol In Vitro, 105746 (2023)

PubMed: 38043628

Applications

WB

Reactivity

Human

Condic, Mateja et al. N6-methyladenosine RNA modification (m6A) is of prognostic value in HPV-dependent vulvar squamous cell carcinoma BMC Cancer, 22, 943 (2022)

PubMed: 36050747

Applications

IHC

Reactivity

Human

Western blot analysis of extracts of various cell lines using METTL3 antibody

Immunohistochemical staining of human lung cancer tissue using METTL3 antibody (dilution of 1:100)

Immunohistochemical staining of human liver tissue using METTL3 antibody (dilution of 1:100)

Immunohistochemical staining of human stomach tissue using METTL3 antibody (dilution of 1:100)

Immunoprecipitation analysis of 150ug extracts of 293T cells using 3ug METTL3 antibody