METTL14 Antibody

• Catalog Number: orb1520323
• Category: Antibodies
• Description: METTL14 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: between 25 and 75
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 50-100 µl/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: METTL14
• UniProt ID: Q9HCE5
• NCBI: Q9HCE5.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: hMETTL14; methyltransferase-like protein 14; N6-ad
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human METTL14 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, RKO, GaMG, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-METTL14 antibody (orb1520323) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of mouse METTL14 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-METTL14 antibody (orb1520323) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human METTL14 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-METTL14 antibody (orb1520323) used for IP at 1 µg per reaction.