Methylene Blue trihydrate

SKU: orb1311034

Description

Methylene Blue trihydrate (Basic Blue 9) is a dark green crystalline powder yielding deep blue aqueous solutions. As a research tool, it functions as a biological stain, a guanylyl cyclase inhibitor, and an agent to counteract cyanide poisoning and methemoglobinemia. In vitro studies also show it inhibits tau protein aggregation, relevant to neurodegenerative disease research.

Research Area

Immunology & Inflammation, Infectious Disease & Virology, Neuroscience, Pharmacology & Drug Discovery, Signal Transduction

Key Properties

• CAS Number: 7220-79-3
• MW: 373.9
• Purity: 99.60%
• Formula: C₁₆H₂₄ClN₃O₃S
• SMILES: O.O.O.[Cl-].CN(C)c1ccc2nc3ccc(cc3[s+]c2c1)N(C)C
• Target: Microtubule Associated,NO Synthase,Parasite,Monoamine Oxidase,Guanylate cyclase
• Solubility: DMSO:3.75 mg/mL (10.03 mM)

Bioactivity

• Target IC50:
  Tau filament:1.9μM
• Cell Research:
  Instructions 1. Cell and tissue staining 1. Dissolve methylene blue in an appropriate solvent (such as water or buffer) to prepare a dye solution of a certain concentration. 2. Soak the cells or tissue sections in the dye solution, usually the staining time is several minutes. 3. After cleaning, observe the cell structure or distribution of target molecules under a microscope. 2. As MAO-A inhibitor and antidepressant and anti-anxiety drugs Methods: Through animal models or cell culture systems, the regulatory effect on neurotransmitters was studied. III. Research on neurodegenerative diseases Methods: Through animal models and cell models, its neuroprotective effects were studied.

Storage & Handling

• Storage: store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

dye, cGMP, Basic blue 9, C.I. Basic Blue 9, anxiolytic, antinociception, antimalarial, antidepressant, inhibit, Monoamine Oxidase, MonoamineOxidase, NOSynthase, NOS, Inhibitor, Nitric oxide synthases, NO Synthase, Methylene Blue, Methylene Blue Trihydrate, Methylene Blue trihydrate, Methylthionine chloride, Methylthionine Chloride, MicrotubuleAssociated, Microtubule Associated, methemoglobinemias, Parasite, Guanylatecyclase, Guanylate Cyclase, Guanylate cyclase, MAO, MAO-A, Tau aggregation, sGC, vasopressor

Compound Identifiers

PubChem CID

104827