Methylene Blue

SKU: orb1300374

Description

Methylene Blue

Research Area

Immunology & Inflammation, Infectious Disease & Virology, Neuroscience, Pharmacology & Drug Discovery, Signal Transduction

Key Properties

• CAS Number: 61-73-4
• MW: 319.85
• Purity: 96.82%
• Formula: C₁₆H₁₈ClN₃S
• SMILES: [Cl-].CN(C)c1ccc2nc3cc\c(cc3sc2c1)=[N+](/C)C
• Target: Microtubule Associated,Monoamine Oxidase,Parasite,Guanylate cyclase,NO Synthase
• Solubility: H2O:3.2 mg/mL (10 mM);DMSO:1 mg/mL (3.13 mM)

Bioactivity

• Target IC50:
  GC:|Tau aggregation:1.9 μM
• In Vivo:
  Methylene Blue decreases C-GMP and vascular smooth muscle relaxation by inhibiting guanylate cyclase. Pharmacokinetic studies in rodents showed that MB injects by intravascular resulting in a rapid and extensive accumulation of this drug in the central nervous system (CNS). When MB is injected intravenously, it selectively gathers in parathyroid glands, therefore is easy for the identification of structures during surgery.
• In Vitro:
  MB could inactivate nitric oxide directly by generating superoxide anions, it may inhibit the action of nitric oxide synthase itself. In addition to interfering with the nitric oxide–cyclic guanosine monophosphate pathway, there are MB directly activating calcium-dependent potassium channels and enhancing the release of noradrenaline from intracellular stores.
• Cell Research:
  Immature neuroblasts, isolated from the newborn rat subventricular zone, differentiate into γ-aminobutyric acid–mediated neurons are exposed at the sixth day in vitro to MB (10 μM) for 2h. Assess the survival and dendritic arbor architecture of these cells 48h after this treatment paradigm.
• Animal Research:
  MB (5 or 50 mg/kg) diluted in normal saline were injected intraarterially into Sprague- Dawley male rats over the 30s.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tau aggregation, Tetramethylthionine Chloride, Tetramethylthionine chloride, vasopressor, MAO, Inhibitor, Guanylate Cyclase, guanylate cyclase, Guanylate cyclase, Guanylatecyclase, inhibit, MonoamineOxidase, Monoamine Oxidase, NOS, NOSynthase, Parasite, methylthioninium Chloride, Methylthioninium, methylthioninium chloride, Methylene Blue, methemoglobinemias, MicrotubuleAssociated, Microtubule Associated, NO Synthase, Nitric oxide synthases, antidepressant, antinociception, antimalarial, anxiolytic, Basic Blue 9, Basic Blue9, Basic Blue-9, dye, cGMP, CI52015, CI-52015, CI 52015

Compound Identifiers

PubChem CID

6099