METAP1 Antibody

• Catalog Number: orb1520808
• Category: Antibodies
• Description: METAP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 336 and 386
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 20 µl/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: METAP1
• UniProt ID: P53582
• NCBI: P53582.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: MetAP1A; methionine aminopeptidase 1; metAP 1; MAP
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse METAP1 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-METAP1 antibody (orb1520808) used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human METAP1 by western blot. Samples: Whole cell lysate (10 µg) from Jurkat, HeLa, HEK293T, MCF-7, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-METAP1 antibody (orb1520808) used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human METAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-METAP1 antibody (orb1520808) used for IP at 2 µg per reaction.