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Metallothionein Antibody: HRP
Catalog Number: orb413724
| Catalog Number | orb413724 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody against Metallothionein-1 Conjugated to HRP |
| Target | Metallothionein |
| Clonality | Monoclonal |
| Species/Host | Mouse |
| Isotype | IgG1 |
| Conjugation | HRP |
| Reactivity | Bacteria |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O |
| Immunogen | Recombinant full length Pseudomonas aeruginosa Metallothionein protein |
| UniProt ID | Q9I1X5 |
| MW | 36 kDa |
| Tested applications | ELISA, IHC, WB |
| Dilution range | WB (1:1000); ELISA (1:800) |
| Application notes | A 1:1000 dilution of SMC-553 was sufficient for detection of Metallothionein in 1 µg of purified Pseudomonas aeruginosa Metallothionein (PmtA)-GST tagged protein by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
| Clone Number | 8D8 |
| Storage | Conjugated antibodies should be stored according to the product label |
| Alternative names | Metallothionein antibody, CES 1 antibody, CES1 ant Read more... |
| Note | For research use only |
| Entrez | 880550 |
| NCBI | NP_250830.1 |
Western Blot analysis of Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged showing detection of 36 kDa Metallothionein protein using Mouse Anti-Metallothionein Monoclonal Antibody, Clone 8D8. Lane 1: Molecular Weight Ladder (MW). Lane 2: GST. Lane 3: Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged. Load: 1 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Metallothionein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 36 kDa.
