Metallothionein Antibody: HRP

• Catalog Number: orb413724
• Category: Antibodies
• Description: Mouse monoclonal antibody against Metallothionein-1 Conjugated to HRP
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 8D8
• Tested applications: ELISA, IHC, WB
• Reactivity: Bacteria
• Isotype: IgG1
• Immunogen: Recombinant full length Pseudomonas aeruginosa Metallothionein protein
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000); ELISA (1:800)
• Conjugation: HRP
• MW: 36 kDa
• Target: Metallothionein
• Entrez: 880550
• UniProt ID: Q9I1X5
• NCBI: NP_250830.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Alternative names: Metallothionein antibody, CES 1 antibody, CES1 ant
• Note: For research use only
• Application notes: A 1:1000 dilution of SMC-553 was sufficient for detection of Metallothionein in 1 µg of purified Pseudomonas aeruginosa Metallothionein (PmtA)-GST tagged protein by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged showing detection of 36 kDa Metallothionein protein using Mouse Anti-Metallothionein Monoclonal Antibody, Clone 8D8. Lane 1: Molecular Weight Ladder (MW). Lane 2: GST. Lane 3: Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged. Load: 1 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Metallothionein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 36 kDa.