Metadherin antibody

• Catalog Number: orb1474245
• Category: Antibodies
• Description: Mouse monoclonal antibody to Metadherin
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: FC, IH, WB
• Reactivity: Human
• Immunogen: Purified recombinant fragment of human Metadherin expressed in E. Coli.
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), FC (1/50 - 1/100)
• Form/Appearance: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Conjugation: Unconjugated
• Target: MTDH
• Entrez: 92140
• UniProt ID: Q86UE4
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Alternative names: AEG1; LYRIC; Protein LYRIC; 3D3/LYRIC; Astrocyte e
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Metadherin expression in Jurkat (A), A549 (B), MCF7 (C), Hela (D) whole cell lysates. (Predicted band size: 64 kD; Observed band size: 64 kD)

Immunohistochemical analysis of Metadherin staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.71). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Flow cytometric analysis of Metadherin in Hela cells (green) and negative control (purple).