Mesothelin/MSLN Antibody

• Catalog Number: orb234333
• Category: Antibodies
• Description: Mesothelin/MSLN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IHC, IHC-Fr, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Mesothelin recombinant protein (Position: K306-L576). Human Mesothelin shares 57% and 56% amino acid (aa) sequence identity with mouse and rat Mesothelin, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: ELISA , 0.1-0.5μg/ml, Human, -Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By HeatWestern blot, 0.1-0.5μg/ml, HumanImmunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry, 0.5-1μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 68986 MW
• UniProt ID: Q13421
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Mesothelin;CAK1 antigen;Pre-pro-megakaryocyte-pote
• Note: For research use only
• Application notes: WB: The detection limit for Mesothelin is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-Mesothelin antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Mesothelin using anti-Mesothelin antibody.Lane 1:Recombinant Human Mesothelin Protein 0.5ng.

WB analysis of Mesothelin using anti-Mesothelin antibody.Lane 1:human HeLa cell.

IHC analysis of Mesothelin using anti-Mesothelin antibody.Mesothelin was detected in paraffin-embedded section of Human Lung Cancer Tissue.