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Catalog Number | orb1954675 |
---|---|
Category | Proteins |
Description | Mesothelin Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 28.2 kDa and the accession number is Q13421-2. |
Tag | C-His |
Purity | 98.00% |
MW | 28.2 kDa (predicted) |
UniProt ID | Q13421 |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | Mesothelin Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 28.2 kDa and the accession number is Q13421-2. |
Expression Region | A DNA sequence encoding the MSLN (Q13421-2) (Met1-Arg286) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro. Predicted N terminal: Leu 37 |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |
98.00% | |
35.44 kDa (predicted); 44.42 kDa (reducing conditions) |
98.00% | |
35.2 kDa (predicted). Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
Biotinylated Recombinant Human Mesothelin Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Glu296-Gly580) of Human Mesothelin fused with His Tag and Avi tag at the C-terminal. |