Mesothelin Antibody / MSLN

• Catalog Number: orb750309
• Category: Antibodies
• Description: Mouse monoclonal antibody against Mesothelin conjugated to Biotin
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MSLN/2131
• Tested applications: IHC-P
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 273-407 from the human protein was used as the immunogen for the Mesothelin antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 2-4ug/ml for 30 minutes at RT
• Purity: Protein G affinity chromatography
• Conjugation: Biotin
• Formula: 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Mesothelin antibody is available for research use only.
• UniProt ID: Q13421
• Storage: Store the Mesothelin antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term).
• Buffer/Preservatives: 0.1 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Mesothelin antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human endometrial carcinoma with biotinylated Mesothelin antibody (clone MSLN/2131). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung with biotinylated Mesothelin antibody (clone MSLN/2131). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Mesothelin antibody (clone MSLN/2131) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Mesothelin antibody (clone MSLN/2131). These results demonstrate the foremost specificity of the MSLN/2131 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.