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Catalog Number | orb1231474 |
---|---|
Category | Proteins |
Description | MERS S Recombinant Protein RBD |
Tested applications | ELISA, WB |
Tag | His Tag |
Form/Appearance | Lyophilized |
Purity | > 90% as determined by SDS-PAGE. |
MW | 28.2 kDa |
Protein Sequence | Glu 367 - Tyr 606 |
Source | HEK293 cells |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS, pH 7.4 |
Alternative names | Spike,S protein RBD,Spike glycoprotein Receptor-bi Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 28.2 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
MERS S protein RBD, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human DPPIV, Fc Tag at 5 ug/ml (100 uL/well) can bind MERS S protein RBD, His Tag with a linear range of 0.6-5 ng/mL.
Immobilized MERS S protein RBD, His Tag at 5 ug/ml (100 uL/well) can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.313-2.5 ug/ml.
Greater than 90.0% as determined by SDS-PAGE. | |
Sf9, Baculovirus cells |
Greater than 90.0% as determined by SDS-PAGE. | |
Sf9, Baculovirus cells |