You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb18300 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to MELK |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Bovine, Canine, Human, Mouse |
Dilution range | ELISA: 1:32000, WB: 0.1-0.3 μg/ml |
Conjugation | Unconjugated |
MW | 74.6 |
Target | MELK |
Entrez | 9833 |
Protein Sequence | KRLVEDILSSCKV |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti MELK antibody, anti maternal embryonic leucin Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Experiments gave bands at approx 75kDa and 150kDa in human testis lysates and in cell lines HeLa and Jurkat after 0.1 μg/ml antibody staining. The150kDa band is explained by glycosylation of this member of the AMPK family. This protein has a calculated MW of 74.6kDa according to NP_055606.1. Recommended concentration: 0.1-0.3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of human testis lysate using MELK antibody
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Primate, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating