MEK7/MAP2K7 Antibody

• Catalog Number: orb334496
• Category: Antibodies
• Description: MEK7/MAP2K7 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human MEK7 (2-40aa AASSLEQKLSRLEAKLKQENREARRRIDLNLDISPQRPR), identical to the related mouse and rat sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.25μg/ml, Human, Monkey, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 47485 MW
• UniProt ID: O14733
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Dual specificity mitogen-activated protein kinase
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U87 cells using anti-MEK7/MAP2K7 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of MEK7/MAP2K7 using anti-MEK7/MAP2K7 antibody.Lane 1:human HeLa cell; 2:monkey COS-7 cell; 3:human Jurkat cell; 4:human Raji cell; 5:rat testis tissue; 7:mouse testis tissue.

IF analysis of MEK7/MAP2K7 using anti-MEK7/MAP2K7 antibody. MEK7/MAP2K7 was detected in an immunocytochemical section of PC-3 cells.

IHC analysis of MEK7/MAP2K7 using anti-MEK7/MAP2K7 antibody. MEK7/MAP2K7 was detected in a paraffin-embedded section of human testicular cancer tissue.