MEK1, Phospho (T286) Antibody

• Catalog Number: orb1524512
• Category: Antibodies
• Description: MEK1, Phospho (T286) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human, Mouse
• Immunogen: surrounding threonine 286
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended; however this product has been shown to perform in western blot on immunoprecipitated MEK1 (i.e. IP with A302-139A, A303-610A, or A303-611A).; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: MEK1, Phospho (T286)
• UniProt ID: Q02750
• NCBI: NP_002746.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: MAPKK1; protein kinase, mitogen-activated, kinase
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Phospho-MEK1 (T286) by immunoprecipitation (IP). Samples: 50 µg HeLa whole cell lysate (WCL) from mock treated (-) or nocodazole treated (+) cells. 1 mg of HeLa WCL mock treated (-) or nocodazole treated (+) and immunoprecipitated; 20% of the IP was loaded. Antibodies: Affinity purified rabbit anti-phospho MEK1 (T286) orb1524512 was used for IP (6ug/1mg lysate). To detect total MEK1, Rabbit anti-MEK1 was used for western blot (WB) at 1 µg/ml.

Detection of human Phospho-MEK1 (T286) by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-phospho MEK1 (T286) (Cat. No. orb1524512) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Detection of mouse Phospho-MEK1 (T286) by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-phospho MEK1 (T286) (Cat. No. orb1524512) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB