MEK-1 (phospho Thr286) rabbit pAb

• Catalog Number: orb769698
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MEK-1 (phospho-Thr286)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Immunogen: The antiserum was produced against synthesized peptide derived from human MEK1 around the phosphorylation site of Thr286. AA range:252-301
• MW: 43
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-MAP2K1 antibody, anti-MEK1 antibody, anti-PRK
• Note: For research use only

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MEK1 (Phospho-Thr286) Antibody.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEK1 (Phospho-Thr286) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from K562 cells, using MEK1 (Phospho-Thr286) Antibody. The lane on the right is blocked with the phospho peptide.