MEK-1 (phospho Ser298) rabbit pAb

• Catalog Number: orb769696
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MEK-1 (phospho-Ser298)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Immunogen: The antiserum was produced against synthesized peptide derived from human MEK1 around the phosphorylation site of Ser298. AA range:264-313
• MW: 45
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-MAP2K1 antibody, anti-MEK1 antibody, anti-PRK
• Note: For research use only

Western blot analysis of HEPG2-UV using p-MEK-1 (S298) antibody. Antibody was diluted at 1:500.

Western blot analysis of lysates from NIH/3T3 cells treated with PDGF 50ng/ml 20', using MEK1 (Phospho-Ser298) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).