MEK 1 Antibody (Phospho-Thr386)

• Catalog Number: orb2192151
• Category: Antibodies
• Description: MEK 1 Antibody (Phospho-Thr386)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr386 of human MEK 1.
• Form/Appearance: Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml rAlbumin and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Conjugation: Unconjugated
• MW: ~45kDa
• UniProt ID: Q02750
• NCBI: NP_002746
• Storage: For long term storage -2°C is recommended. Stable at -2°C for at least 1 year.
• Buffer/Preservatives: Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml rAlbumin and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Alternative names: MEL, CFC3, MEK1, MKK1, MAPKK1, PRKMK1
• Note: For research use only
• Application notes: Application Info: The antibody has been directly tested for reactivity in Western blots with rat and human tissue. It is anticipated that the antibody will react with bovine, canine, chicken, mouse, non-human primates and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen.Western blot of human T47D cells showing specific immunolabeling of the ~45k MEK 1 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda phosphatase: lambda Ptase) The blot is identical to the control except that it was incubated in lambda Ptase (1200 units for 30 min) before being exposed to the MEK 1 Thr386 antibody. The immunolabeling of MEK 1 is completely eliminated by lambda Ptase.
• Expiration Date: 12 months from date of receipt.