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Catalog Number | orb371687 |
---|---|
Category | Antibodies |
Description | MEF2A Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IF, IHC, WB |
Predicted Reactivity | Hamster |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human MEF2A (466-507aa DGSDREDPRGDFHSPIVLGRPPNTEDRESPSVKRMRMDAW VT), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 54811 MW |
UniProt ID | Q02078 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Myocyte-specific enhancer factor 2A;Serum response Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of MEF2A using anti-MEF2A antibody.Lane 1:rat thymus tissue; 2:HELA cell.
IF analysis of MEF2A using anti-MEF2A antibody. MEF2A was detected in immunocytochemical section of U20S cells.
IHC analysis of MEF2A using anti-MEF2A antibody.MEF2A was detected in a paraffin-embedded section of human meningeoma tissue.
IHC analysis of MEF2A using anti-MEF2A antibody.MEF2A was detected in a paraffin-embedded section of rat brain tissue.
IHC analysis of MEF2A using anti-MEF2A antibody.MEF2A was detected in a paraffin-embedded section of mouse brain tissue.
IHC analysis of MEF2A using anti-MEF2A antibody.MEF2A was detected in a paraffin-embedded section of human glioma tissue.
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
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