MED8 Antibody

• Catalog Number: orb402577
• Category: Antibodies
• Description: MED8 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human MED8 recombinant protein (Position: M1-R195).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 29 kDa
• UniProt ID: Q96G25
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Mediator of RNA polymerase II transcription subuni
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of MED8 using anti-MED8 antibody.Lane 1:human THP-1 cell; 2:human HepG2 cell; 3:human U251 cell; 4:human Caco-2 cell; 5:rat brain tissue; 6:rat liver tissue; 7:mouse brain tissue; 8:mouse liver tissue.

IF analysis of MED8 and Tubulin beta using anti-MED8 antibody and anti-Tubulin beta antibody. MED8 and Tubulin beta were detected in immunocytochemical section of U87 cells.

IHC analysis of MED8 using anti-MED8 antibody.MED8 was detected in paraffin-embedded section of mouse testis tissue.

IHC analysis of MED8 using anti-MED8 antibody.MED8 was detected in paraffin-embedded section of rat testis tissue.