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Catalog Number | orb402591 |
---|---|
Category | Antibodies |
Description | MED18 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human MED18 recombinant protein (Position: M1-M208). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 24 kDa |
UniProt ID | Q9BUE0 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Mediator of RNA polymerase II transcription subuni Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of A431 cells using anti-MED18 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of MED18 using anti-MED18 antibody.Lane 1:human A431 cell;2:human K562 cell;3:human U2OS cell;4:human HL-60 cell;5:rat liver tissue;6:mouse kidney tissue.
IF analysis of MED18 using anti-MED18 antibody. MED18 was detected in immunocytochemical section of A431 cells.
IHC analysis of MED18 using anti-MED18 antibody.MED18 was detected in paraffin-embedded section of human intestinal cancer tissue.
IHC analysis of MED18 using anti-MED18 antibody.MED18 was detected in paraffin-embedded section of human lung cancer tissue.
IHC analysis of MED18 using anti-MED18 antibody.MED18 was detected in paraffin-embedded section of mouse brain tissue.
IHC analysis of MED18 using anti-MED18 antibody.MED18 was detected in paraffin-embedded section of mouse small intestine tissue.
IHC analysis of MED18 using anti-MED18 antibody.MED18 was detected in paraffin-embedded section of rat brain tissue.
IHC analysis of MED18 using anti-MED18 antibody.MED18 was detected in paraffin-embedded section of rat small intestine tissue.
IHC analysis of MED18 using anti-MED18 antibody.MED18 was detected in paraffin-embedded section of human mammary cancer tissue.
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