MED13L Antibody

• Catalog Number: orb1526658
• Category: Antibodies
• Description: MED13L Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 550 and 600
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 to 1:1000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: MED13L
• UniProt ID: Q71F56
• NCBI: NP_056150.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: thyroid hormone receptor-associated protein 2; thy
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human MED13L by western blot. Samples: Whole cell lysate (15 µg) from HeLa, GaMG, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-MED13L antibody orb1526658 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human MED13L by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-MED13L antibody orb1526658 used for IP at 6 µg per reaction.

Detection of human MED13L by immunohistochemistry.Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-MED13L used at 1: 1, 000 (0.2µg/ml).Detection: DAB