MED1 Antibody

• Catalog Number: orb1529627
• Category: Antibodies
• Description: MED1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 1525 and C-term
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: MED1
• UniProt ID: Q15648
• NCBI: NP_004765.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: PPAR-binding protein; PPARBP; PPARGBP; RB18A; Thyr
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human MED1 by western blot.Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, MCF-7, and Hep-G2 cells. Antibody: Affinity purified rabbit anti-MED1 antibody at 0.1 µg/ml.

Detection of mouse MED1 by western blot.Samples: Whole cell lysate from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-MED1 antibody at 0.1 µg/ml.

Detection of human MED1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MED1 antibody used at 1:5000 (0.2µg/ml). Secondary:HRP-conjugated goat anti-rabbit IgG.

Detection of human MED1 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-MED1 antibody.