MDM2 Antibody

• Catalog Number: orb2643547
• Category: Antibodies
• Description: MDM2 is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53. It can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. Overexpression of MDM2 protein is detected in a variety of cancers.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SMP14
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: Amino acids 154-167 were used as the immunogen for the MDM2 antibody.
• Antibody Type: Primary Antibody
• Dilution range: ELISA (order BSA-free format for coating),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This MDM2 antibody is available for research use only.
• UniProt ID: Q00987
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human HepG2 cell lysate with MDM2 antibody (clone SMP14). Predicted molecular weight: ~55 kDa but can be observed at up to ~90 kDa.

IHC staining of FFPE human breast cancer with MDM2 antibody (clone SMP14). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free MDM2 antibody (clone SMP14) as confirmation of integrity and purity.