MDC1 Antibody (HRP)

SKU: orb148044

Description

Mouse monoclonal to MDC1 (HRP). MDC1, mediator of DNA damage checkpoint protein 1, plays a role in checkpoint mediated cell cycle arrest in response to DNA damage, within S phase and G2/M. It is also thought to act as a scaffold protein during recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage that are marked by phosphorylation of histone H2A. X on S139..

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution Range: WB (1:2000), ICC/IF (1:100)
• Reactivity: Bovine, Human, Mouse, Primate
• Application Notes:
  0.5 µg/ml was sufficient for detection of MDC1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: P2B11
• Immunogen: GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus
• Target: MDC1
• Molecular Weight: 184kDa
• Purification: Protein G Purified
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MDC1, Nuclear factor with BRCT domains1, Mediator of DNA damage checkpoint 1

Quick Database Links

Gene Symbol

MDC1

UniProt

Q5PSV9

NCBI Gene ID

240087

RefSeq (Protein):NP_001010833.2