Mcpt4 antibody

• Catalog Number: orb20160
• Category: Antibodies
• Description: Goat polyclonal antibody to Mcpt4
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Mouse
• Dilution range: ELISA: 1:64000, WB: 0.01 μg/ml, IHC-P: 3.75 μg/ml
• Conjugation: Unconjugated
• MW: 27.2
• Target: Mcpt4 / Myonase (mouse)
• Protein Sequence: KLQKKAKETPSVN
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti Mcpt4 antibody, anti mast cell protease 4 ant
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave bands at approx. 40kDa and 15kDa in Mouse Skeletal Muscle lysates after 0.01 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 27.2kDa according to NP_034909.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Mouse Skeletal Muscle using Mcpt4 antibody