MCP-1 Antibody [2.2-4H5-01A11]

• Catalog Number: orb1272246
• Category: Antibodies
• Description: MCP-1 Antibody [2.2-4H5-01A11]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2.2-4H5-01A11
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant hMCP-1/MCAF as the immunizing antigen.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: CCL2
• UniProt ID: P13500
• NCBI: P13500
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MCP1, SCYA2, C-C motif chemokine 2, HC11, MCAF, MC
• Note: For research use only
• Application notes: ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 300 pg/mL of recombinant human MCP-1 when used with our biotinylated antigen affinity purified anti-human MCP-1 (XP-5218Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot:To detect hMCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1 is 1.0-2.0 ng/lane, under reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 ug/ml of this antibody will detect at least 300 pg/ml of recombinant human MCP-1 when used with antigen affinity purified anti-human MCP-1 (orb1272403) as the detection antibody at a concentration of approximately 0.5-1.0 ug/ml.

To detect hMCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1 is 1.0-2.0 ng/lane, under reducing or non-reducing conditions.

To detect hMCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1 is 1.0-2.0 ng/lane, under reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.