MCM7 Monoclonal Antibody

• Catalog Number: orb547820
• Category: Antibodies
• Description: MCM7 Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: BA-13
• Tested applications: FC, ICC, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human MCM7 Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit.
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Dilution range: WB 1:500-1:2000IHC 1:50-1:200ICC/IF 1:50-1:200IP 1:50FC 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• UniProt ID: P33993
• Storage: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MCM7 expression in HeLa cell lysate using anti-MCM7 antibody (orb547820). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM7 antigen affinity purified polyclonal antibody (Catalog # orb547820) at 0.5 ug/mL overnight at 4.