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Catalog Number | orb1530561 |
---|---|
Category | Antibodies |
Description | MCM5 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | between 1 and 50 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:5,000 - 1:20,000; IP - 2 - 4 µg/mg lysate. This antibody is efficient at pulling down the MCM2-7 complex.; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | MCM5 |
UniProt ID | P33992 |
NCBI | NP_006730.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | minichromosome maintenance deficient 5 (cell divis Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human MCM5 by western blot.Samples: Whole cell lysate from HeLa and Jurkat cells. Antibody: Affinity purified rabbit anti-MCM5 antibody orb1530561 at 0.05 µg/ml.
Detection of human and mouse MCM5 by immunohistochemistry. Sample: FFPE sections of human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-MCM5 used at a dilution of 1:1, 000 (1µg/ml).
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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