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Catalog Number | orb1937365 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB31108 |
Tested applications | FC, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 107673 Da |
Target | This MCF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 804-833 amino acids from the C-terminal region of human MCF2. |
UniProt ID | P10911 |
NCBI | NP_001165350.1, NP_001165348.1, NP_005360.3, NP_001093325.1, NP_001165349.1, NP_001165347.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Proto-oncogene DBL, Proto-oncogene MCF-2, MCF2-tra Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
MCF2 Antibody (C-term) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the MCF2 antibody detected the MCF2 protein (arrow).
MCF2 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
MCF2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MCF2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.